• Western blot analysis of extracts of THP-1 cells, using YKL-40/CHI3L1 antibody (STJ23123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using YKL-40/CHI3L1 antibody (STJ23123) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using YKL-40/CHI3L1 antibody (STJ23123) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using YKL-40/CHI3L1 antibody (STJ23123) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of RAW264. 7 cells using YKL-40/CHI3L1 rabbit polyclonal antibody (STJ23123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using YKL-40/CHI3L1 rabbit polyclonal antibody (STJ23123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CHI3L1 antibody (22-383) (STJ23123)

SKU:
STJ23123

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-YKL-40/CHI3L1 (22-383) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHI3L1
Gene ID: 1116
Uniprot ID: CH3L1_HUMAN
Immunogen Region: 22-383
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-383 of human YKL-40/CHI3L1 (NP_001267.2).
Immunogen Sequence: YKLVCYYTSWSQYREGDGSC FPDALDRFLCTHIIYSFANI SNDHIDTWEWNDVTLYGMLN TLKNRNPNLKTLLSVGGWNF GSQRFSKIASNTQSRRTFIK SVPPFLRTHGFDGLDLAWLY PGRRDKQHFTTLIKEMKAEF IKEAQPGKKQLLLSAALSAG KVTIDSSYDIAKISQHLDFI SIMTYDFHGAWRGTTGHHS
Tissue Specificity Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
Post Translational Modifications Glycosylated.
Function Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Protein Name Chitinase-3-Like Protein 1
39 Kda Synovial Protein
Cartilage Glycoprotein 39
Cgp-39
Gp-39
Hcgp-39
Ykl-40
Database Links Reactome: R-HSA-6798695
Cellular Localisation Secreted
Extracellular Space
Cytoplasm
Perinuclear Region
Endoplasmic Reticulum
Alternative Antibody Names Anti-Chitinase-3-Like Protein 1 antibody
Anti-39 Kda Synovial Protein antibody
Anti-Cartilage Glycoprotein 39 antibody
Anti-Cgp-39 antibody
Anti-Gp-39 antibody
Anti-Hcgp-39 antibody
Anti-Ykl-40 antibody
Anti-CHI3L1 antibody

Information sourced from Uniprot.org

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