| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CEP131 (1000-1083) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CEP131 |
| Gene ID: |
22994 |
| Uniprot ID: |
CP131_HUMAN |
| Immunogen Region: |
1000-1083 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1000-1083 of human CEP131 (NP_001306157.1). |
| Immunogen Sequence: |
IRQEFEDRLAASEEETRQAK AELATLQARQQLELEEVHRR VKTALARKEEAVSSLRTQHE AAVKRADHLEELLEQHRRPT PSTK |
| Post Translational Modifications | Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation. Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation. MAPKAPK2-dependent phosphorylation at Ser-47 and Ser-78 occurs in response to cellular stress such as exposure to ultraviolet irradiation and promotes binding to 14-3-3 proteins which leads to cytoplasmic sequestration of CEP131 and blocks formation of new centriolar satellites. Phosphorylation at Ser-78 mediated by PLK4 is essential for proper organization and integrity of centriolar satellites but is dispensable for its localization to centrioles and its function in ciliogenesis. |
| Function | Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation. In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner. Acts also as a negative regulator of BBSome ciliary trafficking. Plays a role in sperm flagellar formation.may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail. Required for optimal cell proliferation and cell cycle progression.may play a role in the regulation of genome stability in non-ciliogenic cells. Involved in centriole duplication. Required for CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. Essential for maintaining proper centriolar satellite integrity. |
| Protein Name | Centrosomal Protein Of 131 Kda5-Azacytidine-Induced Protein 1Pre-Acrosome Localization Protein 1 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentriolar SatelliteCentrioleCilium Basal BodyCytoplasmic VesicleSecretory VesicleAcrosomeColocalized With Pericentriolar Material Protein Pcm1 At Centriolar SatellitesDuring SpermiogenesisBecomes Enriched With Nephrocystin Nphp1 At The Transition ZoneA Structure At The Base Of The Ciliary Axoneme Important For Regulating Traffic Into The Ciliary CompartmentTraffics Towards And Away From The Centrosome/Basal Body And The Transition Zone Of The Ciliary Axoneme In A Microtubule-Dependent MannerLocalized At The Golgi-Derived Acrosome And The Centrosome-Containing Head-Tail Coupling Apparatus (Htca)Ubiquitinated Form Is Sequestered And Colocalized With Bbs4Cep290Pcm1 And Pcnt At Centriolar Satellites In Proliferating CellsColocalized With The Pericentriolar Material Protein Pcm1 At CentrosomeTraffics Towards And Away From Centriolar Satellites And Centrosome In A Microtubule- And Dynein-Dependent Manner In Interphase CellsDisplaced From Centriolar Satellites But Still Remains Associated With The Centrosome In Response To Cellular StressSuch As Ultraviolet Light (Uv) Radiation Or Heat ShockIn A Process That Requires P38 Mapk Signaling |
| Alternative Antibody Names | Anti-Centrosomal Protein Of 131 Kda antibodyAnti-5-Azacytidine-Induced Protein 1 antibodyAnti-Pre-Acrosome Localization Protein 1 antibodyAnti-CEP131 antibodyAnti-AZI1 antibodyAnti-KIAA1118 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
