• Western blot analysis of extracts of various cell lines, using CDC5L rabbit monoclonal antibody (STJ11102482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CDC5L Rabbit monoclonal antibody (STJ11102482) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using CDC5L rabbit monoclonal antibody (STJ11102482) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using CDC5L Rabbit monoclonal antibody (STJ11102482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDC5L antibody (300-400) [S2MR] (STJ11102482)

SKU:
STJ11102482

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CDC5L (300-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC5L
Gene ID: 988
Uniprot ID: CDC5L_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CDC5L (Q99459).
Immunogen Sequence: PQISDAELQEVVKVGQASEI ARQTAEESGITNSASSTLLS EYNVTNNSVALRTPRTPASQ DRILQEAQNLMALTNVDTPL KGGLNTPLHESDFSGVTPQR Q
Tissue Specificity Ubiquitously expressed in both fetal and adult tissues.
Post Translational Modifications Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
Function DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Protein Name Cell Division Cycle 5-Like Protein
Cdc5-Like Protein
Pombe Cdc5-Related Protein
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
May Shuttle Between Cytoplasm And Nucleus
Alternative Antibody Names Anti-Cell Division Cycle 5-Like Protein antibody
Anti-Cdc5-Like Protein antibody
Anti-Pombe Cdc5-Related Protein antibody
Anti-CDC5L antibody
Anti-KIAA0432 antibody
Anti-PCDC5RP antibody

Information sourced from Uniprot.org

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