| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CD71 (89-763) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Tfrc |
| Gene ID: |
22042 |
| Uniprot ID: |
TFR1_MOUSE |
| Immunogen Region: |
89-763 |
| Immunogen: |
Recombinant Protein corresponding to a sequence within amino acids 89-763 of mouse CD71 (NP_035768.1). |
| Immunogen Sequence: |
CKRVEQKEECVKLAETEETD KSETMETEDVPTSSRLYWAD LKTLLSEKLNSIEFADTIKQ LSQNTYTPREAGSQKDESLA YYIENQFHEFKFSKVWRDEH YVKIQVKSSIGQNMVTIVQS NGNLDPVESPEGYVAFSKPT EVSGKLVHANFGTKKDFEEL SYSVNGSLVIVRAGEITFAE KVANAQSFNAIGVLIYMDKN KFPVVEADLALFGHAHLGTG DPYTPGFPSFNHTQFPPSQ |
| Post Translational Modifications | Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation. Stearoylation does not affect iron uptake. N- and O-glycosylated, phosphorylated and palmitoylated. |
| Function | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Upon stimulation, positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2. |
| Protein Name | Transferrin Receptor Protein 1TrTfrTfr1TrfrCd Antigen Cd71 |
| Database Links | Reactome: R-MMU-432722Reactome: -MMU-8856825Reactome: -MMU-8856828Reactome: -MMU-8980692Reactome: -MMU-9013026Reactome: -MMU-9013106Reactome: -MMU-9013149Reactome: -MMU-9013404Reactome: -MMU-9013406Reactome: -MMU-9013407Reactome: -MMU-9013408Reactome: -MMU-9013423Reactome: -MMU-917977Reactome: -MMU-9696270Reactome: -MMU-9696273 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinMelanosome |
| Alternative Antibody Names | Anti-Transferrin Receptor Protein 1 antibodyAnti-Tr antibodyAnti-Tfr antibodyAnti-Tfr1 antibodyAnti-Trfr antibodyAnti-Cd Antigen Cd71 antibodyAnti-Tfrc antibodyAnti-Trfr antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
