• Western blot analysis of extracts of various cell lines, using CD40L antibody (STJ11101557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD40LG antibody (50-150) [S7MR] (STJ11101557)

SKU:
STJ11101557

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD40L (50-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD40LG
Gene ID: 959
Uniprot ID: CD40L_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CD40L (P29965).
Immunogen Sequence: LDKIEDERNLHEDFVFMKTI QRCNTGERSLSLLNCEEIKS QFEGFVKDIMLNKEETKKEN SFEMQKGDQNPQIAAHVISE ASSKTTSVLQWAEKGYYTMS N
Tissue Specificity Specifically expressed on activated CD4+ T-lymphocytes.
Post Translational Modifications The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated.
Function Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3.both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Protein Name Cd40 Ligand
Cd40-L
T-Cell Antigen Gp39
Tnf-Related Activation Protein
Trap
Tumor Necrosis Factor Ligand Superfamily Member 5
Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form
Scd40l
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-5668541
Reactome: R-HSA-5676594
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Cell Surface
Cd40 Ligand
Soluble Form: Secreted
Release Of Soluble Cd40l From Platelets Is Partially Regulated By Gp Iib/Iiia
Actin Polymerization
And A Matrix Metalloproteinases (Mmp) Inhibitor-Sensitive Pathway
Alternative Antibody Names Anti-Cd40 Ligand antibody
Anti-Cd40-L antibody
Anti-T-Cell Antigen Gp39 antibody
Anti-Tnf-Related Activation Protein antibody
Anti-Trap antibody
Anti-Tumor Necrosis Factor Ligand Superfamily Member 5 antibody
Anti-Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form antibody
Anti-Scd40l antibody
Anti-CD40LG antibody
Anti-CD40L antibody
Anti-TNFSF5 antibody
Anti-TRAP antibody

Information sourced from Uniprot.org

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