• Immunofluorescence analysis of paraffin-embedded Human placenta tissue using CD34 Rabbit polyclonal antibody (STJ11105652) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of CD34 in paraffin-embedded human kidney tissue using CD34 Rabbit polyclonal antibody (STJ11105652) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CD34 in paraffin-embedded human liver cancer tissue using CD34 Rabbit polyclonal antibody (STJ11105652) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CD34 in paraffin-embedded human pancreas tissue using CD34 Rabbit polyclonal antibody (STJ11105652) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CD34 in paraffin-embedded human spleen tissue using CD34 Rabbit polyclonal antibody (STJ11105652) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from TF-1 cells using CD34 Rabbit polyclonal antibody (STJ11105652) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:30s.

Anti-CD34 antibody (32-290) (STJ11105652)

SKU:
STJ11105652

€140.63 - €653.13
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Hematopoietic Progenitor Cell antigen Cd34 (32-290) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD34
Gene ID: 947
Uniprot ID: CD34_HUMAN
Immunogen Region: 32-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 32-290 of human CD34 (NP_001020280.1).
Immunogen Sequence: SLDNNGTATPELPTQGTFSN VSTNVSYQETTTPSTLGSTS LHPVSQHGNEATTNITETTV KFTSTSVITSVYGNTNSSVQ SQTSVISTVFTTPANVSTPE TTLKPSLSPGNVSDLSTTST SLATSPTKPYTSSSPILSDI KAEIKCSGIREVKLTQGICL EQNKTSSCAEFKKDRGEGLA RVLCGEEQADADAGAQVCSL LLAQSEVRPQCLLLVLANRT EISSKLQLMKKHQSDLKKL
Tissue Specificity Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Post Translational Modifications Highly glycosylated. Phosphorylated on serine residues by PKC.
Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Protein Name Hematopoietic Progenitor Cell Antigen Cd34
Cd Antigen Cd34
Database Links Reactome: R-HSA-198933 P28906-1
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Hematopoietic Progenitor Cell Antigen Cd34 antibody
Anti-Cd Antigen Cd34 antibody
Anti-CD34 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance