| Host: |
Mouse |
| Applications: |
IHC-P/FC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CD221 IGF Receptor is suitable for use in Immunohistochemistry, Flow Cytometry and Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
1H7 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with azide |
| Purification: |
Purified |
| Dilution Range: |
IHC-P 1:10-1:40FC 1:10WB |
| Storage Instruction: |
Store at 2-8°C upon receipt. |
| Immunogen: |
Purified human placental CD221 IGF-1 receptor |
| Background | IGF-1 Receptor recognizes human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting (1). CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signaling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (1). Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Pretreatment: protein digestion (Trypsin or Pronase) is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
