• Western blot analysis of various lysates, using CD146 Rabbit polyclonal antibody (STJ11105192) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-CD146 antibody (STJ11105192)

SKU:
STJ11105192

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD146 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mcam
Gene ID: 84004
Uniprot ID: MUC18_MOUSE
Immunogen: Recombinant protein of mouse CD146.
Tissue Specificity Detected in melanoma cell lines.
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Protein Name Cell Surface Glycoprotein Muc18
Gicerin
Melanoma Cell Adhesion Molecule
Melanoma-Associated Antigen Muc18
Cd Antigen Cd146
Database Links Reactome: R-MMU-8980692
Reactome: -MMU-9013026
Reactome: -MMU-9013106
Reactome: -MMU-9013149
Reactome: -MMU-9013404
Reactome: -MMU-9013405
Reactome: -MMU-9013408
Reactome: -MMU-9013423
Reactome: -MMU-9035034
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cell Surface Glycoprotein Muc18 antibody
Anti-Gicerin antibody
Anti-Melanoma Cell Adhesion Molecule antibody
Anti-Melanoma-Associated Antigen Muc18 antibody
Anti-Cd Antigen Cd146 antibody
Anti-Mcam antibody
Anti-Muc18 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance