• Western blot analysis of various lysates, using CD146/MCAM antibody (STJ11102800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CD146/CD146/MCAM antibody (STJ11102800) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD146/MCAM antibody (340-560) (STJ11102800)

SKU:
STJ11102800

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD146/MCAM (340-560) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCAM
Gene ID: 4162
Uniprot ID: MUC18_HUMAN
Immunogen Region: 340-560
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-560 of human CD146/CD146/MCAM (NP_006491.2).
Immunogen Sequence: VNYVSDVRVSPAAPERQEGS SLTLTCEAESSQDLEFQWLR EETGQVLERGPVLQLHDLKR EAGGGYRCVASVPSIPGLNR TQLVNVAIFGPPWMAFKERK VWVKENMVLNLSCEASGHPR PTISWNVNGTASEQDQDPQR VLSTLNVLVTPELLETGVEC TASNDLGKNTSILFLELVNL TTLTPDSNTTTGLSTSTASP HTRANSTSTERKLPEPESRG V
Tissue Specificity Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Protein Name Cell Surface Glycoprotein Muc18
Cell Surface Glycoprotein P1h12
Melanoma Cell Adhesion Molecule
Melanoma-Associated Antigen A32
Melanoma-Associated Antigen Muc18
S-Endo 1 Endothelial-Associated Antigen
Cd Antigen Cd146
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013408
Reactome: R-HSA-9013423
Reactome: R-HSA-9035034
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cell Surface Glycoprotein Muc18 antibody
Anti-Cell Surface Glycoprotein P1h12 antibody
Anti-Melanoma Cell Adhesion Molecule antibody
Anti-Melanoma-Associated Antigen A32 antibody
Anti-Melanoma-Associated Antigen Muc18 antibody
Anti-S-Endo 1 Endothelial-Associated Antigen antibody
Anti-Cd Antigen Cd146 antibody
Anti-MCAM antibody
Anti-MUC18 antibody

Information sourced from Uniprot.org

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