• Flow cytometry: 1X10^6 CHO cells (negative control, left) and CHO (Transfection, right) cells were surface-stained with CD138/Syndecan-1 Rabbit monoclonal antibody (STJ11105293, 2 ug/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (5 Mu l/Test, blue line) , followed by Alexa Fluor® 647 conjugated goat anti-rabbit polyclonal antibody staining. Non-fluorescently stained cells were used as blank control (red line).
  • Flow cytometry: 1X10^6 CHO (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (5 Mu l/Test, left) or CD138/Syndecan-1 Rabbit monoclonal antibody (STJ11105293, 2 ug/mL, right).
  • Confocal imaging of paraffin-embedded Rat lung tissue using CD138/Syndecan-1 Rabbit monoclonal antibody (STJ11105293, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Mouse lung tissue using CD138/Syndecan-1 Rabbit monoclonal antibody (STJ11105293, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Mouse liver tissue using CD138/Syndecan-1 Rabbit monoclonal antibody (STJ11105293, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-CD138/Syndecan-1 antibody (23-255) [S5293RM] (STJ11105293)

SKU:
STJ11105293

£146.50 - £639.50
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Host: Rabbit
Applications: FC
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD138/Syndecan-1 (23-255) is suitable for use in Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S5293RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: FC 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Sdc1
Gene ID: 20969
Uniprot ID: SDC1_MOUSE
Immunogen Region: 23-255
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-255 of mouse CD138/Syndecan-1 (NP_035649.1).
Immunogen Sequence: QIVAVNVPPEDQDGSGDDSD NFSGSGTGALPDTLSRQTPS TWKDVWLLTATPTAPEPTSS NTETAFTSVLPAGEKPEEGE PVLHVEAEPGFTARDKEKEV TTRPRETVQLPITQRASTVR VTTAQAAVTSHPHGGMQPGL HETSAPTAPGQPDHQPPRVE GGGTSVIKEVVEDGTANQLP AGEGSGEQDFTFETSGENTA VAAVEPGLRNQPPVDEGATG ASQSLLDRKEVLG
Post Translational Modifications Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
Function Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP. Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway.
Protein Name Syndecan-1
Synd1
Cd Antigen Cd138
Database Links Reactome: R-MMU-1971475
Reactome: -MMU-2022928
Reactome: -MMU-2024096
Reactome: -MMU-202733
Reactome: -MMU-3000170
Reactome: -MMU-449836
Reactome: -MMU-975634
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Secreted
Extracellular Exosome
Shedding Of The Ectodomain Produces A Soluble Form
Alternative Antibody Names Anti-Syndecan-1 antibody
Anti-Synd1 antibody
Anti-Cd Antigen Cd138 antibody
Anti-Sdc1 antibody
Anti-Synd-1 antibody
Anti-Synd1 antibody

Information sourced from Uniprot.org

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