• Western blot analysis of extracts of A-431 cells, using CCR2 antibody (STJ11100106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of Raw264 7 cells using CCR2 rabbit polyclonal antibody (STJ11100106) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using CCR2 rabbit polyclonal antibody (STJ11100106) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U937 cells using CCR2 rabbit polyclonal antibody (STJ11100106) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Anti-CCR2 antibody (140-240) (STJ11100106)

SKU:
STJ11100106

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCR2 (140-240) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCR2
Gene ID: 729230
Uniprot ID: CCR2_HUMAN
Immunogen Region: 140-240
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 140-240 of human CCR2 (NP_001116513.2).
Immunogen Sequence: LAIVHAVFALKARTVTFGVV TSVITWLVAVFASVPGIIFT KCQKEDSVYVCGPYFPRGWN NFHTIMRNILGLVLPLLIMV ICYSGILKTLLRCRNEKKRH R
Tissue Specificity Expressed by monocytes and IL2-activated NK cells.
Post Translational Modifications N-glycosylated. Sulfation increases the affinity for both monomeric and dimeric CCL2 with stronger binding to the monomeric form. Binding of sulfated CCR2 to CCL2 promotes conversion of CCL2 from dimer to monomer.
Function Key functional receptor for CCL2 but can also bind CCL7 and CCL12. Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (Probable). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B. Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation. Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression.signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B. Mediates the recruitment of macrophages and monocytes to the injury site following brain injury. (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
Protein Name C-C Chemokine Receptor Type 2
C-C Ckr-2
Cc-Ckr-2
Ccr-2
Ccr2
Monocyte Chemoattractant Protein 1 Receptor
Mcp-1-R
Cd Antigen Cd192
Database Links Reactome: R-HSA-1461957
Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-6783783
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
The Chemoattractant Receptors Are Distributed Throughout The Cell Surface
After Stimulation With A Ligand
Such As Ccl2
They Are Rapidly Recruited Into Microdomain Clusters At The Cell Membrane
Alternative Antibody Names Anti-C-C Chemokine Receptor Type 2 antibody
Anti-C-C Ckr-2 antibody
Anti-Cc-Ckr-2 antibody
Anti-Ccr-2 antibody
Anti-Ccr2 antibody
Anti-Monocyte Chemoattractant Protein 1 Receptor antibody
Anti-Mcp-1-R antibody
Anti-Cd Antigen Cd192 antibody
Anti-CCR2 antibody
Anti-CMKBR2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance