• Immunohistochemistry analysis of paraffin-embedded mouse testis using CBX6 antibody (STJ27484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using CBX6 antibody (STJ27484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using CBX6 antibody (STJ27484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of HeLa cells, using CBX6 antibody (STJ27484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CBX6 antibody (80-180) (STJ27484)

SKU:
STJ27484

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CBX6 (80-180) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBX6
Gene ID: 23466
Uniprot ID: CBX6_HUMAN
Immunogen Region: 80-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-180 of human CBX6 (NP_055107.3).
Immunogen Sequence: LLKARAQAEALRISDVHFSV KPSASASSPKLHSSAAVHRL KKDIRRCHRMSRRPLPRPDP QGGSPGLRPPISPFSETVRI INRKVKPREPKRNRIILNLK V
Post Translational Modifications Ubiquitinated. Ubiquitination regulates the function of the Polycomb group (PcG) multiprotein PRC1-like complex. Deubiquitinated by USP26.
Function Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin. Recruitment to chromatin might occur in an H3K27me3-independent fashion. May have a PRC1-independent function in embryonic stem cells.
Protein Name Chromobox Protein Homolog 6
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-8939243
Reactome: R-HSA-8943724
Cellular Localisation Nucleus
Chromosome
Uniformely Distributed In The Nucleoplasm
Localizes To The Inactivated X Chromosome In Females
Alternative Antibody Names Anti-Chromobox Protein Homolog 6 antibody
Anti-CBX6 antibody

Information sourced from Uniprot.org

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