• Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Catalase antibody (STJ11101296) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Catalase Rabbit monoclonal antibody (STJ11101296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CAT antibody (1-100) [S6MR] (STJ11101296)

SKU:
STJ11101296

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Catalase (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAT
Gene ID: 847
Uniprot ID: CATA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Catalase (P04040).
Immunogen Sequence: MADSRDPASDQMQHWKEQRA AQKADVLTTGAGNPVGDKLN VITVGPRGPLLVQDVVFTDE MAHFDRERIPERVVHAKGAG AFGYFEVTHDITKYSKAKVF
Function Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Protein Name Catalase
Database Links Reactome: R-HSA-3299685
Reactome: R-HSA-6798695
Reactome: R-HSA-9033241
Reactome: R-HSA-9615017
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Catalase antibody
Anti-CAT antibody

Information sourced from Uniprot.org

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