• Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using CA8 antibody (STJ29682) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CA8 antibody (STJ29682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CA8 antibody (1-290) (STJ29682)

SKU:
STJ29682

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CA8 (1-290) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA8
Gene ID: 767
Uniprot ID: CAH8_HUMAN
Immunogen Region: 1-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human CA8 (NP_004047.3).
Immunogen Sequence: MADLSFIEDTVAFPEKEEDE EEEEEGVEWGYEEGVEWGLV FPDANGEYQSPINLNSREAR YDPSLLDVRLSPNYVVCRDC EVTNDGHTIQVILKSKSVLS GGPLPQGHEFELYEVRFHWG RENQRGSEHTVNFKAFPMEL HLIHWNSTLFGSIDEAVGKP HGIAIIALFVQIGKEHVGLK AVTEILQDIQYKGKSKTIPC FNPNTLLPDPLLRDYWVYEG SLTIPPCSEGVTWILFRYP
Function Does not have a carbonic anhydrase catalytic activity.
Protein Name Carbonic Anhydrase-Related Protein
Carp
Carbonic Anhydrase Viii
Ca-Viii
Alternative Antibody Names Anti-Carbonic Anhydrase-Related Protein antibody
Anti-Carp antibody
Anti-Carbonic Anhydrase Viii antibody
Anti-Ca-Viii antibody
Anti-CA8 antibody
Anti-CALS antibody

Information sourced from Uniprot.org

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