• Western blot analysis of extracts of various cell lines, using C5AR1 antibody (STJ116164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using C5AR1 antibody (STJ116164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human appendix using C5AR1 rabbit polyclonal antibody (STJ116164) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-C5AR1 antibody (1-282) (STJ116164)

SKU:
STJ116164

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C5AR1 (1-282) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: C5AR1
Gene ID: 728
Uniprot ID: C5AR1_HUMAN
Immunogen Region: 1-282
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human C5AR1 (NP_001727.2).
Immunogen Sequence: MDSFNYTTPDYGHYDDKDTL DLNTPVDKTSNTLRVPDILA LVIFAVVFLVGVLGNALVVW VTAFEAKRTINAIWFLNLAV ADFLSCLALPILFTSIVQHH HWPFGGAACSILPSLILLNM YASILLLATISADRFLLVFK PIWCQNFRGAGLAWIACAVA WGLALLLTIPSFLYRVVREE YFPPKVLCGVDYSHDKRRER AVAIVRLVLGFLWPLLTLTI CYTFILLRTWSRRATRSTK
Post Translational Modifications Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist. Sulfation at Tyr-14 is important for CHIPS binding. Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338.
Function Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.
Protein Name C5a Anaphylatoxin Chemotactic Receptor 1
C5a Anaphylatoxin Chemotactic Receptor
C5a-R
C5ar
Cd Antigen Cd88
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-6798695
Reactome: R-HSA-977606
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle
Phosphorylated C5ar Colocalizes With Arrb1 And Arrb2 In Cytoplasmic Vesicles
Alternative Antibody Names Anti-C5a Anaphylatoxin Chemotactic Receptor 1 antibody
Anti-C5a Anaphylatoxin Chemotactic Receptor antibody
Anti-C5a-R antibody
Anti-C5ar antibody
Anti-Cd Antigen Cd88 antibody
Anti-C5AR1 antibody
Anti-C5AR antibody
Anti-C5R1 antibody

Information sourced from Uniprot.org

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