| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BRSK2 (200-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BRSK2 |
| Gene ID: |
9024 |
| Uniprot ID: |
BRSK2_HUMAN |
| Immunogen Region: |
200-300 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human BRSK2 (NP_001243556.1). |
| Immunogen Sequence: |
WSCGVILFALLVGALPFDDD NLRQLLEKVKRGVFHMPHFI PPDCQSLLRGMIEVDAARRL TLEHIQKHIWYIGGKNEPEP EQPIPRKVQIRSLPSLEDID P |
| Tissue Specificity | Detected in pancreas islets (at protein level). |
| Post Translational Modifications | Phosphorylated at Thr-174 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-174 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-174 by PP2C. Phosphorylated at Thr-260 by PKA. Phosphorylation at Thr-260 by PKA was not observed in another study, but this may reflect differences in the experimental approach. Phosphorylation at Thr-260 seems to play a role in the regulation of insulin secretion. Polyubiquitinated by the APC complex in conjunction with FZR1, leading to its proteasomal degradation. Targeted for proteasomal degradation by interaction with COPS5. BRSK2 levels change during the cell cycle. BRSK2 levels are low at the G1/S boundary and gradually increase as cells progress into G2 phase. BRSK2 levels decrease rapidly at the end of mitosis. |
| Function | Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion, BRSK2 phosphorylated at Thr-260 can promote insulin secretion. Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress. |
| Protein Name | Serine/Threonine-Protein Kinase Brsk2Brain-Selective Kinase 2Brain-Specific Serine/Threonine-Protein Kinase 2Br Serine/Threonine-Protein Kinase 2Serine/Threonine-Protein Kinase 29Serine/Threonine-Protein Kinase Sad-A |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomePerinuclear RegionEndoplasmic ReticulumDetected At Centrosomes During MitosisLocalizes To The Endoplasmic Reticulum In Response To Stress Caused By Tunicamycin |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Brsk2 antibodyAnti-Brain-Selective Kinase 2 antibodyAnti-Brain-Specific Serine/Threonine-Protein Kinase 2 antibodyAnti-Br Serine/Threonine-Protein Kinase 2 antibodyAnti-Serine/Threonine-Protein Kinase 29 antibodyAnti-Serine/Threonine-Protein Kinase Sad-A antibodyAnti-BRSK2 antibodyAnti-C11orf7 antibodyAnti-PEN11B antibodyAnti-SADA antibodyAnti-STK29 antibodyAnti-HUSSY-12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
