• Immunohistochemistry analysis of paraffin-embedded rat brain using BRD2 Rabbit polyclonal antibody (STJ118680) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using BRD2 Rabbit polyclonal antibody (STJ118680) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of HeLa cells, using BRD2 antibody (STJ118680) at 1:1000 dilution. HeLa cells were treated by NaCl (400mM) Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of mouse brain, using BRD2 antibody (STJ118680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BRD2 antibody (301-400) (STJ118680)

SKU:
STJ118680

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRD2 (301-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRD2
Gene ID: 6046
Uniprot ID: BRD2_HUMAN
Immunogen Region: 301-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 301-400 of human BRD2 (NP_005095.1).
Immunogen Sequence: SPPGSLEPKAARLPPMRRES GRPIKPPRKDLPDSQQQHQS SKKGKLSEQLKHCNGILKEL LSKKHAAYAWPFYKPVDASA LGLHDYHDIIKHPMDLSTVK
Function May play a role in spermatogenesis or folliculogenesis. Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.
Protein Name Bromodomain-Containing Protein 2
O27.1.1
Really Interesting New Gene 3 Protein
Database Links Reactome: R-HSA-8951936
Cellular Localisation Nucleus
Detected On Chromatin And Nucleosomes
Alternative Antibody Names Anti-Bromodomain-Containing Protein 2 antibody
Anti-O27.1.1 antibody
Anti-Really Interesting New Gene 3 Protein antibody
Anti-BRD2 antibody
Anti-KIAA9001 antibody
Anti-RING3 antibody

Information sourced from Uniprot.org

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