| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BRCC3 (1-316) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BRCC3 |
| Gene ID: |
79184 |
| Uniprot ID: |
BRCC3_HUMAN |
| Immunogen Region: |
1-316 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human BRCC3 (NP_077308.1). |
| Immunogen Sequence: |
MAVQVVQAVQAVHLESDAFL VCLNHALSTEKEEVMGLCIG ELNDDTRSDSKFAYTGTEMR TVAEKVDAVRIVHIHSVIIL RRSDKRKDRVEISPEQLSAA STEAERLAELTGRPMRVVGW YHSHPHITVWPSHVDVRTQA MYQMMDQGFVGLIFSCFIED KNTKTGRVLYTCFQSIQAQK SSESLHGPRDFWSSSQHISI EGQKEEERYERIEIPIHIVP HVTIGKVCLESAVELPKIL |
| Tissue Specificity | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors. |
| Function | Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1.deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Acts as a regulator of the NLRP3 inflammasome by mediating deubiquitination of NLRP3, leading to NLRP3 inflammasome assembly. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination. Deubiquitinates HDAC1 and PWWP2B leading to their stabilization. |
| Protein Name | Lys-63-Specific Deubiquitinase Brcc36Brca1-A Complex Subunit Brcc36Brca1/Brca2-Containing Complex Subunit 3Brca1/Brca2-Containing Complex Subunit 36Brisc Complex Subunit Brcc36 |
| Database Links | Reactome: R-HSA-5689901Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-69473 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle PoleLocalizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs)Interaction With Abraxas2 Retains Brcc3 In The Cytoplasm |
| Alternative Antibody Names | Anti-Lys-63-Specific Deubiquitinase Brcc36 antibodyAnti-Brca1-A Complex Subunit Brcc36 antibodyAnti-Brca1/Brca2-Containing Complex Subunit 3 antibodyAnti-Brca1/Brca2-Containing Complex Subunit 36 antibodyAnti-Brisc Complex Subunit Brcc36 antibodyAnti-BRCC3 antibodyAnti-BRCC36 antibodyAnti-C6.1A antibodyAnti-CXorf53 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89077/357534/STJ11100004_1__87524.1713121649.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
