• Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AKR1B1 antibody (1-316) (STJ11100004)

SKU:
STJ11100004

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AKR1B1 (1-316) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKR1B1
Gene ID: 231
Uniprot ID: ALDR_HUMAN
Immunogen Region: 1-316
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human AKR1B1 (NP_001619.1).
Immunogen Sequence: MASRLLLNNGAKMPILGLGT WKSPPGQVTEAVKVAIDVGY RHIDCAHVYQNENEVGVAIQ EKLREQVVKREELFIVSKLW CTYHEKGLVKGACQKTLSDL KLDYLDLYLIHWPTGFKPGK EFFPLDESGNVVPSDTNILD TWAAMEELVDEGLVKAIGIS NFNHLQVEMILNKPGLKYKP AVNQIECHPYLTQEKLIQYC QSKGIVVTAYSPLGSPDRPW AKPEDPSLLEDPRIKAIAA
Tissue Specificity Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
Function Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls).
Protein Name Aldo-Keto Reductase Family 1 Member B1
Aldehyde Reductase
Aldose Reductase
Ar
Database Links Reactome: R-HSA-196108
Reactome: R-HSA-5652227
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Aldo-Keto Reductase Family 1 Member B1 antibody
Anti-Aldehyde Reductase antibody
Anti-Aldose Reductase antibody
Anti-Ar antibody
Anti-AKR1B1 antibody
Anti-ALDR1 antibody
Anti-ALR2 antibody

Information sourced from Uniprot.org

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