• Western blot analysis of lysates from Jurkat and 293 cells, using BCKD Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 3T3 cells using BCKDK Polyclonal Antibody
  • Immunofluorescence analysis of HeLa cells, using BCKD Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using BCKDK Polyclonal Antibody

Anti-BCKDK antibody (11-60 aa) (STJ91832)

SKU:
STJ91832

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-3-methyl-2-oxobutanoate dehydrogenase kinase, mitochondrial (11-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCKDK
Gene ID: 10295
Uniprot ID: BCKD_HUMAN
Immunogen Region: 11-60 aa
Specificity: BCKDK Polyclonal Antibody detects endogenous levels of BCKDK protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BCKD at the amino acid range 11-60
Post Translational Modifications Autophosphorylated.
Function Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
Protein Name 3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial
Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase
Bckd-Kinase
Bckdhkin
Database Links Reactome: R-HSA-70895
Cellular Localisation Mitochondrion Matrix
Mitochondrion
Alternative Antibody Names Anti-3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial antibody
Anti-Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase antibody
Anti-Bckd-Kinase antibody
Anti-Bckdhkin antibody
Anti-BCKDK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance