• Western blot analysis of extracts of various cell lines, using ATP2A3 antibody (STJ116445) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-ATP2A3 antibody (360-600) (STJ116445)

SKU:
STJ116445

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP2A3 (360-600) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP2A3
Gene ID: 489
Uniprot ID: AT2A3_HUMAN
Immunogen Region: 360-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-600 of human ATP2A3 (NP_005164.2).
Immunogen Sequence: QMSVCRMFVVAEADAGSCLL HEFTISGTTYTPEGEVRQGD QPVRCGQFDGLVELATICAL CNDSALDYNEAKGVYEKVGE ATETALTCLVEKMNVFDTDL QALSRVERAGACNTVIKQLM RKEFTLEFSRDRKSMSVYCT PTRPHPTGQGSKMFVKGAPE SVIERCSSVRVGSRTAPLTP TSREQILAKIRDWGSGSDTL RCLALATRDAPPRKEDMELD DCSKFVQYETDLTFVGCVG
Tissue Specificity Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. Expressed in cell lineages of hematopoietic, epithelial, or embryonic origin and also expressed in several cancer cell lines.
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Protein Name Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 3
Serca3
Sr Ca(2+-Atpase 3
Calcium Pump 3
Database Links Reactome: R-HSA-1912420
Reactome: R-HSA-418359
Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Cellular Localisation Nucleus Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Sarcoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 3 antibody
Anti-Serca3 antibody
Anti-Sr Ca(2+-Atpase 3 antibody
Anti-Calcium Pump 3 antibody
Anti-ATP2A3 antibody

Information sourced from Uniprot.org

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