| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-APEX2 (1-180) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
APEX2 |
| Gene ID: |
27301 |
| Uniprot ID: |
APEX2_HUMAN |
| Immunogen Region: |
1-180 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APEX2 (NP_055296.2). |
| Immunogen Sequence: |
MLRVVSWNINGIRRPLQGVA NQEPSNCAAVAVGRILDELD ADIVCLQETKVTRDALTEPL AIVEGYNSYFSFSRNRSGYS GVATFCKDNATPVAAEEGLS GLFATQNGDVGCYGNMDEFT QEELRALDSEGRALLTQHKI RTWEGKEKTLTLINVYCPHA DPGRPERLVFKMRFYRLLQI |
| Tissue Specificity | Highly expressed in brain and kidney. Weakly expressed in the fetal brain. |
| Function | Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Also displays double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Also exhibits 3'-5' exonuclease activity on a single nucleotide gap containing heteroduplex DNA and on blunt-ended substrates. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Plays a role in reversing blocked 3' DNA ends, problematic lesions that preclude DNA synthesis. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. |
| Protein Name | Dna-(Apurinic Or Apyrimidinic Site Endonuclease 2Ap Endonuclease Xth2Apex Nuclease 2Apex Nuclease-Like 2Apurinic-Apyrimidinic Endonuclease 2Ap Endonuclease 2 |
| Cellular Localisation | NucleusCytoplasmMitochondrionTogether With PcnaIs Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents |
| Alternative Antibody Names | Anti-Dna-(Apurinic Or Apyrimidinic Site Endonuclease 2 antibodyAnti-Ap Endonuclease Xth2 antibodyAnti-Apex Nuclease 2 antibodyAnti-Apex Nuclease-Like 2 antibodyAnti-Apurinic-Apyrimidinic Endonuclease 2 antibodyAnti-Ap Endonuclease 2 antibodyAnti-APEX2 antibodyAnti-APE2 antibodyAnti-APEXL2 antibodyAnti-XTH2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
