| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Ap-2 Complex Subunit Beta is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5219RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AP2B1 |
| Gene ID: |
163 |
| Uniprot ID: |
AP2B1_HUMAN |
| Immunogen: |
Recombinant protein of human AP2B1. |
| Tissue Specificity | Expressed in the brain (at protein level). |
| Post Translational Modifications | Phosphorylation at Tyr-737 by SRC occurs at the plasma membrane in clathrin-coated vesicles (CCVs). |
| Function | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins.at least some clathrin-associated sorting proteins (CLASPs) are recognized by their DE-X(1,2)-F-X-X-FL-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly.clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly. |
| Protein Name | Ap-2 Complex Subunit BetaAp105bAdaptor Protein Complex Ap-2 Subunit BetaAdaptor-Related Protein Complex 2 Subunit BetaBeta-2-AdaptinBeta-AdaptinClathrin Assembly Protein Complex 2 Beta Large ChainPlasma Membrane Adaptor Ha2/Ap2 Adaptin Beta Subunit |
| Database Links | Reactome: R-HSA-167590Reactome: R-HSA-177504Reactome: R-HSA-182218Reactome: R-HSA-2132295Reactome: R-HSA-3928665Reactome: R-HSA-416993 P63010-1Reactome: R-HSA-437239Reactome: R-HSA-5099900Reactome: R-HSA-5140745Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8866427Reactome: R-HSA-8964038Reactome: R-HSA-9679191 |
| Cellular Localisation | Cell MembraneMembraneCoated PitPeripheral Membrane ProteinCytoplasmic SideAp-2 Appears To Be Excluded From Internalizing Ccvs And To Disengage From Sites Of Endocytosis Seconds Before Internalization Of The Nascent Ccv |
| Alternative Antibody Names | Anti-Ap-2 Complex Subunit Beta antibodyAnti-Ap105b antibodyAnti-Adaptor Protein Complex Ap-2 Subunit Beta antibodyAnti-Adaptor-Related Protein Complex 2 Subunit Beta antibodyAnti-Beta-2-Adaptin antibodyAnti-Beta-Adaptin antibodyAnti-Clathrin Assembly Protein Complex 2 Beta Large Chain antibodyAnti-Plasma Membrane Adaptor Ha2/Ap2 Adaptin Beta Subunit antibodyAnti-AP2B1 antibodyAnti-ADTB2 antibodyAnti-CLAPB1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
