Host: | Rabbit |
Applications: | WB |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit monoclonal antibody anti-Ap-2 Complex Subunit Beta is suitable for use in Western Blot research applications. |
Clonality: | Monoclonal |
Clone ID: | S5219RM |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
Purification: | Affinity purification |
Dilution Range: | WB 1:500-1:1000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | AP2B1 |
Gene ID: | 163 |
Uniprot ID: | AP2B1_HUMAN |
Immunogen: | Recombinant protein of human AP2B1. |
Tissue Specificity | Expressed in the brain (at protein level). |
Post Translational Modifications | Phosphorylation at Tyr-737 by SRC occurs at the plasma membrane in clathrin-coated vesicles (CCVs). |
Function | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins.at least some clathrin-associated sorting proteins (CLASPs) are recognized by their DE-X(1,2)-F-X-X-FL-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly.clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly. |
Protein Name | Ap-2 Complex Subunit Beta Ap105b Adaptor Protein Complex Ap-2 Subunit Beta Adaptor-Related Protein Complex 2 Subunit Beta Beta-2-Adaptin Beta-Adaptin Clathrin Assembly Protein Complex 2 Beta Large Chain Plasma Membrane Adaptor Ha2/Ap2 Adaptin Beta Subunit |
Database Links | Reactome: R-HSA-167590 Reactome: R-HSA-177504 Reactome: R-HSA-182218 Reactome: R-HSA-2132295 Reactome: R-HSA-3928665 Reactome: R-HSA-416993 P63010-1 Reactome: R-HSA-437239 Reactome: R-HSA-5099900 Reactome: R-HSA-5140745 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-8866427 Reactome: R-HSA-8964038 Reactome: R-HSA-9679191 |
Cellular Localisation | Cell Membrane Membrane Coated Pit Peripheral Membrane Protein Cytoplasmic Side Ap-2 Appears To Be Excluded From Internalizing Ccvs And To Disengage From Sites Of Endocytosis Seconds Before Internalization Of The Nascent Ccv |
Alternative Antibody Names | Anti-Ap-2 Complex Subunit Beta antibody Anti-Ap105b antibody Anti-Adaptor Protein Complex Ap-2 Subunit Beta antibody Anti-Adaptor-Related Protein Complex 2 Subunit Beta antibody Anti-Beta-2-Adaptin antibody Anti-Beta-Adaptin antibody Anti-Clathrin Assembly Protein Complex 2 Beta Large Chain antibody Anti-Plasma Membrane Adaptor Ha2/Ap2 Adaptin Beta Subunit antibody Anti-AP2B1 antibody Anti-ADTB2 antibody Anti-CLAPB1 antibody |
Information sourced from Uniprot.org