| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AK4 (1-223) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200IP 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AK4 |
| Gene ID: |
205 |
| Uniprot ID: |
KAD4_HUMAN |
| Immunogen Region: |
1-223 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1). |
| Immunogen Sequence: |
MASKLLRAVILGPPGSGKGT VCQRIAQNFGLQHLSSGHFL RENIKASTEVGEMAKQYIEK SLLVPDHVITRLMMSELENR RGQHWLLDGFPRTLGQAEAL DKICEVDLVISLNIPFETLK DRLSRRWIHPPSGRVYNLDF NPPHVHGIDDVTGEPLVQQE DDKPEAVAARLRQYKDVAKP VIELYKSRGVLHQFSGTETN KIWPYVYTLFSNKITPIQSK EAY |
| Tissue Specificity | Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain. |
| Function | Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity. Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK. Plays a protective role in the cellular response to oxidative stress. |
| Protein Name | Adenylate Kinase 4 - MitochondrialAk 4Adenylate Kinase 3-LikeGtp -Amp Phosphotransferase Ak4 |
| Database Links | Reactome: R-HSA-499943 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Adenylate Kinase 4 - Mitochondrial antibodyAnti-Ak 4 antibodyAnti-Adenylate Kinase 3-Like antibodyAnti-Gtp -Amp Phosphotransferase Ak4 antibodyAnti-AK4 antibodyAnti-AK3 antibodyAnti-AK3L1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89668/358164/STJ11100730_1__42931.1713122467.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
