• Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g AK4 antibody (STJ22562). Western blot was performed from the immunoprecipitate using AK4 antibody (STJ22562) at a dilution of 1:1000.
  • Immunofluorescence analysis of HeLa cells using AK4 Rabbit polyclonal antibody (STJ22562). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using AK4 Rabbit polyclonal antibody (STJ22562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AK4 antibody (1-223) (STJ22562)

SKU:
STJ22562

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AK4 (1-223) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AK4
Gene ID: 205
Uniprot ID: KAD4_HUMAN
Immunogen Region: 1-223
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1).
Immunogen Sequence: MASKLLRAVILGPPGSGKGT VCQRIAQNFGLQHLSSGHFL RENIKASTEVGEMAKQYIEK SLLVPDHVITRLMMSELENR RGQHWLLDGFPRTLGQAEAL DKICEVDLVISLNIPFETLK DRLSRRWIHPPSGRVYNLDF NPPHVHGIDDVTGEPLVQQE DDKPEAVAARLRQYKDVAKP VIELYKSRGVLHQFSGTETN KIWPYVYTLFSNKITPIQSK EAY
Tissue Specificity Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain.
Function Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity. Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK. Plays a protective role in the cellular response to oxidative stress.
Protein Name Adenylate Kinase 4 - Mitochondrial
Ak 4
Adenylate Kinase 3-Like
Gtp -Amp Phosphotransferase Ak4
Database Links Reactome: R-HSA-499943
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Adenylate Kinase 4 - Mitochondrial antibody
Anti-Ak 4 antibody
Anti-Adenylate Kinase 3-Like antibody
Anti-Gtp -Amp Phosphotransferase Ak4 antibody
Anti-AK4 antibody
Anti-AK3 antibody
Anti-AK3L1 antibody

Information sourced from Uniprot.org

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