• Western blot analysis of extracts of various cell lines, using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human brain using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of THP-1 cells using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of paraffin-embedded mouse brain using AIF1/IBA1 rabbit monoclonal antibody (STJ11103127, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat brain using AIF1/IBA1 rabbit monoclonal antibody (STJ11103127, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-AIF1/IBA1 antibody (1-100) [S7MR] (STJ11103127)

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STJ11103127

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-AIF1/IBA1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Aif1
Gene ID: 11629
Uniprot ID: AIF1_MOUSE
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse AIF1/IBA1 (O70200).
Immunogen Sequence: MSQSRDLQGGKAFGLLKAQQ EERLEGINKQFLDDPKYSND EDLPSKLEAFKVKYMEFDLN GNGDIDIMSLKRMLEKLGVP KTHLELKRLIREVSSGSEET
Tissue Specificity Abundantly expressed in the testis, moderately in the spleen and lymph nodes and at low levels in the liver and thymus. Detected in macrophages.
Post Translational Modifications Phosphorylated on serine residues.
Function Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Protein Name Allograft Inflammatory Factor 1
Aif-1
Ionized Calcium-Binding Adapter Molecule 1
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Phagocytic Cup
Associated With The Actin Cytoskeleton At Membrane Ruffles And At Sites Of Phagocytosis
Alternative Antibody Names Anti-Allograft Inflammatory Factor 1 antibody
Anti-Aif-1 antibody
Anti-Ionized Calcium-Binding Adapter Molecule 1 antibody
Anti-Aif1 antibody
Anti-Iba1 antibody

Information sourced from Uniprot.org

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