• Western blot analysis of extracts of various cell lines, using ADPGK antibody (STJ29252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ADPGK antibody (217-496) (STJ29252)

SKU:
STJ29252

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADPGK
Gene ID: 83440
Uniprot ID: ADPGK_HUMAN
Immunogen Region: 217-496
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 217-496 of human ADPGK (NP_112574.3).
Immunogen Sequence: EWGQLKAPHANRFIFSHDLS NGAMNMLEVFVSSLEEFQPD LVVLSGLHMMEGQSKELQRK RLLEVVTSISDIPTGIPVHL ELASMTNRELMSSIVHQVFP AVTSLGLNEQELLFLTQSAS GPHSSLSSWNGVPDVGMVSD ILFWILKEHGRSKSRASDLT RIHFHTLVYHILATVDGHWA NQLAAVAAGARVAGTQACAT ETIDTSRVSLRAPQEFMTSH SEAGSRIVLNPNKPVVEWH
Function Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.
Protein Name Adp-Dependent Glucokinase
Adp-Gk
Adpgk
Rbbp-35
Database Links Reactome: R-HSA-70171
Cellular Localisation Secreted
Alternative Antibody Names Anti-Adp-Dependent Glucokinase antibody
Anti-Adp-Gk antibody
Anti-Adpgk antibody
Anti-Rbbp-35 antibody
Anti-ADPGK antibody
Anti-PSEC0260 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance