• Western blot analysis of extracts of various cell lines, using ADD2 antibody (STJ111528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ADD2 antibody (580-690) (STJ111528)

SKU:
STJ111528

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADD2 (580-690) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADD2
Gene ID: 119
Uniprot ID: ADDB_HUMAN
Immunogen Region: 580-690
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 580-690 of human ADD2 (NP_001171983.1).
Immunogen Sequence: DGEKETAPEEPGSPAKSAPA SPVQSPAKEAETKSPLVSPS KSLEEGTKKTETSKAATTEP ETTQPEGVVVNGREEEQTAE EILSKGLSQMTTSADTDVDT SKDKTESVTSG
Tissue Specificity Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.
Post Translational Modifications The N-terminus is blocked.
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.
Protein Name Beta-Adducin
Erythrocyte Adducin Subunit Beta
Database Links Reactome: R-HSA-5223345
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Beta-Adducin antibody
Anti-Erythrocyte Adducin Subunit Beta antibody
Anti-ADD2 antibody
Anti-ADDB antibody

Information sourced from Uniprot.org

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