• Western blot analysis of extracts of various cell lines, using CHL1 antibody (STJ111786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using CHL1 antibody (STJ111786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHL1 antibody (690-950) (STJ111786)

SKU:
STJ111786

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHL1 (690-950) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHL1
Gene ID: 10752
Uniprot ID: NCHL1_HUMAN
Immunogen Region: 690-950
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 690-950 of human CHL1 (NP_001240316.1).
Immunogen Sequence: NEVGRSQPSQPSDHHETPPA APDRNPQNIRVQASQPKEMI IKWEPLKSMEQNGPGLEYRV TWKPQGAPVEWEEETVTNHT LRVMTPAVYAPYDVKVQAIN QLGSGPDPQSVTLYSGEDYP DTAPVIHGVDVINSTLVKVT WSTVPKDRVHGRLKGYQINW WKTKSLLDGRTHPKEVNILR FSGQRNSGMVPSLDAFSEFH LTVLAYNSKGAGPESEPYIF QTPEGVPEQPTFLKVIKVD
Tissue Specificity Expressed in the fetal and adult brain as well as in Schwann cell culture. Also detected in adult peripheral tissues.
Post Translational Modifications Cleavage by metalloprotease ADAM8 in the extracellular part generates 2 soluble forms (125 kDa and 165 kDa) in vitro and is inhibited by metalloprotease inhibitors. Cleaved by BACE1. N-glycosylated. Contains N-linked oligosaccharides with a sulfated carbohydrate structure type HNK-1 (SO4-3-GlcUABeta1,3GalBeta1,4GlcNAc). O-glycosylated.
Function Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane.
Protein Name Neural Cell Adhesion Molecule L1-Like Protein
Close Homolog Of L1 Cleaved Into - Processed Neural Cell Adhesion Molecule L1-Like Protein
Database Links Reactome: R-HSA-447041
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Soluble Forms Produced By Cleavage/Shedding Also Exist
Processed Neural Cell Adhesion Molecule L1-Like Protein: Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Neural Cell Adhesion Molecule L1-Like Protein antibody
Anti-Close Homolog Of L1 Cleaved Into - Processed Neural Cell Adhesion Molecule L1-Like Protein antibody
Anti-CHL1 antibody
Anti-CALL antibody

Information sourced from Uniprot.org

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