• Immunofluorescence analysis of U2OS cells using ADAP1 antibody (STJ22512) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ADAP1 antibody (STJ22512) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ADAP1 antibody (1-374) (STJ22512)

SKU:
STJ22512

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAP1 (1-374) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAP1
Gene ID: 11033
Uniprot ID: ADAP1_HUMAN
Immunogen Region: 1-374
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADAP1 (NP_006860.1).
Immunogen Sequence: MAKERRRAVLELLQRPGNAR CADCGAPDPDWASYTLGVFI CLSCSGIHRNIPQVSKVKSV RLDAWEEAQVEFMASHGNDA ARARFESKVPSFYYRPTPSD CQLLREQWIRAKYERQEFIY PEKQEPYSAGYREGFLWKRG RDNGQFLSRKFVLTEREGAL KYFNRNDAKEPKAVMKIEHL NATFQPAKIGHPHGLQVTYL KDNSTRNIFIYHEDGKEIVD WFNALRAARFHYLQVAFPG
Tissue Specificity Expressed at highest levels in brain and at lower levels in peripheral blood leukocytes.
Post Translational Modifications Phosphorylated by PRKCA, PRKCI, PRKCZ and PRKD1 in vitro.
Function GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).
Protein Name Arf-Gap With Dual Ph Domain-Containing Protein 1
Centaurin-Alpha-1
Cnt-A1
Putative Mapk-Activating Protein Pm25
Database Links Reactome: R-HSA-1251985
Cellular Localisation Nucleus
Cytoplasm
Recruited To The Plasma Membrane Upon Epidermal Growth Factor-Dependent Activation Of Phosphatidylinositol 4
5-Diphosphate (Ptdinsp2) 3-Kinase
Alternative Antibody Names Anti-Arf-Gap With Dual Ph Domain-Containing Protein 1 antibody
Anti-Centaurin-Alpha-1 antibody
Anti-Cnt-A1 antibody
Anti-Putative Mapk-Activating Protein Pm25 antibody
Anti-ADAP1 antibody
Anti-CENTA1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance