• Western blot analysis of extracts of various cell lines, using ACVRL1 rabbit monoclonal antibody (STJ11102541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Confocal imaging of NIH/3T3 cells using ACVRL1 Rabbit monoclonal antibody (STJ11102541, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of C6 cells using ACVRL1 rabbit monoclonal antibody (STJ11102541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of C6 cells using ACVRL1 Rabbit monoclonal antibody (STJ11102541, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunofluorescence analysis of NIH-3T3 cells using ACVRL1 rabbit monoclonal antibody (STJ11102541) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ACVRL1 Rabbit monoclonal antibody (STJ11102541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ACVRL1 antibody (404-503) [S1MR] (STJ11102541)

SKU:
STJ11102541

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ACVRL1 (404-503) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACVRL1
Gene ID: 94
Uniprot ID: ACVL1_HUMAN
Immunogen Region: 404-503
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 404-503 of human ACVRL1 (P37023).
Immunogen Sequence: VLWEIARRTIVNGIVEDYRP PFYDVVPNDPSFEDMKKVVC VDQQTPTIPNRLAADPVLSG LAQMMRECWYPNPSARLTAL RIKKTLQKISNSPEKPKVIQ
Function Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
Protein Name Serine/Threonine-Protein Kinase Receptor R3
Skr3
Activin Receptor-Like Kinase 1
Alk-1
Tgf-B Superfamily Receptor Type I
Tsr-I
Database Links Reactome: R-HSA-201451
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Receptor R3 antibody
Anti-Skr3 antibody
Anti-Activin Receptor-Like Kinase 1 antibody
Anti-Alk-1 antibody
Anti-Tgf-B Superfamily Receptor Type I antibody
Anti-Tsr-I antibody
Anti-ACVRL1 antibody
Anti-ACVRLK1 antibody
Anti-ALK1 antibody

Information sourced from Uniprot.org

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