| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
YES1 Positive Control for STJ503566 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
YES1 |
| Gene ID: |
7525 |
| Uniprot ID: |
YES_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with YES1 antibody STJ503566. |
| Tissue Specificity | Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis. |
| Post Translational Modifications | Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-426 maintains enzyme activity by blocking CSK-mediated inhibition. Palmitoylation at Cys-3 promotes membrane localization. |
| Function | Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity. |
| Peptide Name | Tyrosine-Protein Kinase YesProto-Oncogene C-YesP61-Yes |
| Database Links | Reactome: R-HSA-1227986Reactome: R-HSA-1433557Reactome: R-HSA-1433559Reactome: R-HSA-2029481Reactome: R-HSA-210990Reactome: R-HSA-2682334Reactome: R-HSA-389356Reactome: R-HSA-389513Reactome: R-HSA-3928662Reactome: R-HSA-3928663Reactome: R-HSA-3928665Reactome: R-HSA-8940973Reactome: R-HSA-912631Reactome: R-HSA-9664323Reactome: R-HSA-9664422Reactome: R-HSA-9670439Reactome: R-HSA-9680350 |
| Cellular Localisation | Cell MembraneCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCytosolNewly Synthesized Protein Initially Accumulates In The Golgi Region And Traffics To The Plasma Membrane Through The Exocytic Pathway |
| Alternative Peptide Names | Tyrosine-Protein Kinase Yes proteinProto-Oncogene C-Yes proteinP61-Yes proteinYES1 proteinYES protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
