Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of various lysates using [KD Validated] RHOT1/MIRO1 Rabbit pAb (STJ28401) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-RHOT1 antibody (310-590) (STJ28401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-RHOT1 antibody (50-100 aa) (STJ13100187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Western blot analysis of extracts of various cell lines, using (STJ25355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RHOT2 antibody (340-550) (STJ25355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-RHOT2 antibody (STJA0006838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from U-87MG cells, using RHOU Rabbit polyclonal antibody (STJ119338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RHOU antibody (40-100) (STJ119338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from mouse stomach, using RHOU Rabbit polyclonal antibody (STJ119339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RHOU antibody (40-100) (STJ119339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-RhoU antibody (Internal Region) (STJ13101914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-RhoU antibody (Internal Region) (STJ13101918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-RHOV antibody (C-Term) (STJ13101916)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-RHOV antibody (STJ13101917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Western blot analysis of A549 cells using Rhophilin Polyclonal Antibody

Anti-RHPN1 antibody (474-523 aa) (STJ95454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-RHPN2 antibody (110-190 aa) (STJ191329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from PC-12 cells, using RICTOR Rabbit polyclonal antibody (STJ11100924) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RICTOR antibody (1043-1708) (STJ11100924)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Anti-RICTOR antibody (STJA0010245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Western blot analysis using Rictor monoclonal antibody against HeLa (1) , PANC-1 (2) , MOLT4 (3) , HepG2 (4) and HEK293 (5) cell lysate.

Anti-RICTOR antibody [7B3] (STJ98362)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Anti-RING1 antibody (STJA0009464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g RING1A antibody (STJ11103112). Western blot was performed from the immunoprecipitate using RING1A antibody (STJ11103112) at a dilution of 1:1000.

Anti-RING1A antibody (100-200) [S2MR] (STJ11103112)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of RING1A in paraffin-embedded mouse stomach using RING1A Rabbit polyclonal antibody (STJ11103202) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-RING1A antibody (207-406) (STJ11103202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using RIPK1/RIP Rabbit polyclonal antibody (STJ29550) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RIPK1 antibody (100-200) (STJ29550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g RIPK1/RIP Rabbit monoclonal antibody (STJ11101710). Western blot was performed from the immunoprecipitate using RIPK1/RIP Rabbit monoclonal antibody (STJ11101710) at a dilution of 1:500.

Anti-RIPK1 antibody (289-490) [S0MR] (STJ11101710)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-RIPK1 antibody (620-670) (STJ502798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-RIPK1 antibody (620-670) {Biotin} (STJ502799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-RIPK1 antibody (620-670) {FITC} (STJ502800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-RIPK1 antibody (STJ191954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-RIPK2 antibody (1-540) (STJ115343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using RIPK2 antibody (STJ25358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RIPK2 antibody (1-540) (STJ25358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 lysis using RIP2 antibody. Antibody was diluted at 1:500

Anti-RIPK2 antibody (146-195 aa) (STJ95512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-RIPK2 antibody (410-460) (STJ502801)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Pig/Monkey

£427.50

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Anti-RIPK2 antibody (410-460) {Biotin} (STJ502802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Pig/Monkey

£528.50

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Anti-RIPK2 antibody (410-460) {FITC} (STJ502803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bovine/Human/Pig/Monkey

£528.50

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Western blot analysis of various cells using Rit1 Polyclonal Antibody diluted at 1：500

Anti-RIT1 antibody (170-219 aa) (STJ95514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using RIT1 Rabbit polyclonal antibody (STJ118161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-RIT1 antibody (C-Term) (STJ118161)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RIT2 antibody (STJ190852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from 22Rv1 cells, using RLN2 Rabbit pAb (STJ29049) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-RLN2 antibody (25-185) (STJ29049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded Human tonsil using Relaxin 2/RLN2 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-RLN2 antibody (25-35 aa) [R05-7A5] (STJA0011897)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RLN2 antibody (STJ192159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-RLN3 antibody (Internal) (STJ95406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RND1 antibody (140-220 aa) (STJ191330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using RND1 Rabbit polyclonal antibody (STJ115660) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RND1 antibody (187-206) (STJ115660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RND2 antibody (140-220 aa) (STJ191334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from 293T cells, using RND3 Rabbit pAb (STJ113242) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-RND3 antibody (145-244) (STJ113242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-RND3 antibody (150-230 aa) (STJ191331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using RNF111 Rabbit polyclonal antibody (STJ11100233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RNF111 antibody (400-500) (STJ11100233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of RNF111 Antibody. The lane on the right is blocked with the RNF111 peptide.

Anti-RNF111 antibody (901-950 aa) (STJ91694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RING1B/RNF2 antibody (STJ27509) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-RNF2 antibody (1-336) (STJ27509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of U-937 cells using 3 Mu g RING1B/RNF2 antibody (STJ11101899). Western blot was performed from the immunoprecipitate using RING1B/RNF2 antibody (STJ11101899) at a dilution of 1:500.

Anti-RNF2 antibody (100-200) [S9MR] (STJ11101899)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using RING2/RING1B/RNF2 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-RNF2 antibody (118-131 aa) [R04-2B3] (STJA0012662)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£264.50

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Anti-RNF2 antibody (STJA0006996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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