Neuronal System | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using AMPK Beta 1 rabbit monoclonal antibody (STJ11102047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PRKAB1 antibody (50-150) [S7MR] (STJ11102047)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot detection of AMPK beta 1 in 3T3, Hela, PC-12, COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.

Anti-PRKAB1 antibody [1A7-E11-E9] (STJ99185)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP/IHC

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Western blot analysis of 1) Jurkat Cell, 2) Mouse Brain, 3) PC12 Cell lysate using AMPK β1 mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-PRKAB1 antibody [Mix] (STJ196664)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various lysates using PRKAB2 Rabbit pAb (STJ115304) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PRKAB2 antibody (1-272) (STJ115304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using PRKAB2 Rabbit polyclonal antibody (STJ29032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAB2 antibody (1-272) (STJ29032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PRKAB2 antibody (130-210) (STJA0010093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 1) 293T, 2) HepG2, 3) Mouse Heart Tissue, 4) Rat Heart Tissue using AMPKβ2 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-PRKAB2 antibody (STJ197441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human LiverTissue using AMPK β2 mouse Monoclonal antibody diluted at 1:200.

Anti-PRKAB2 antibody [Mix] (STJ196665)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various lysates using PKA C-alpha (PRKACA) Rabbit polyclonal antibody (STJ111027) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKACA antibody (1-351) (STJ111027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using PKAC Alpha Rabbit polyclonal antibody (STJ11105351) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKACA antibody (251-351) (STJ11105351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of cAMP Protein Kinase Catalytic subunit (green) in Hela using cAMP Protein Kinase Catalytic subunit antibody, and DAPI (blue)

Anti-PRKACA antibody (319-332 aa) [R07-8H3] (STJA0011064)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£246.50

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Western blot analysis of lysates from wild type (WT) and PKA C-alpha (PRKACA) knockdown (KD) 293T cells, using [KD Validated] PKA C-alpha (PRKACA) Mouse monoclonal antibody (STJ11104590) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRKACA antibody [S4590MM] (STJ11104590)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of Rat spleen, using PKA C-beta (PRKACB) antibody (STJ27277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PRKACB antibody (250-351) (STJ27277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Jurkat and COLO205 cells, using KAPCB Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PRKACB antibody (291-340 aa) (STJ95115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of Rat brain, using PRKACG antibody (STJ110026) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-PRKACG antibody (1-351) (STJ110026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of COLO205 cells using PKA Gamma cat Polyclonal Antibody

Anti-PRKACG antibody (1-50 aa) (STJ95116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of PRKACG in mouse testis, mouse lung lysates using PKA C gamma antibody.

Anti-PRKACG antibody (338-351 aa) [R01-8I1] (STJA0011829)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Immunofluorescence analysis of MCF-7 cells using PRKAG1 antibody (STJ29439). Blue: DAPI for nuclear staining.

Anti-PRKAG1 antibody (1-331) (STJ29439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293 cells using AMPK Gamma 1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PRKAG1 antibody (10-59 aa) (STJ91585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using AMPK Gamma 1 Rabbit polyclonal antibody (STJ11104668) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.

Anti-PRKAG1 antibody (100-200) (STJ11104668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PRKAG1 antibody [3G12] (STJ196666)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human ProstateTissue using AMPK γ1 mouse Monoclonal antibody diluted at 1:200.

Anti-PRKAG1 antibody [5H5] (STJ196661)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of K562 using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-PRKAG2 antibody (1-50 aa) (STJ91587)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-PRKAG3 antibody (1-210) (STJ29606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of RD cells using PRKAG3 Rabbit polyclonal antibody (STJ116067) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAG3 antibody (1-70) (STJ116067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from normal (control) and PRKAR1A knockout (KO) 293T cells, using PRKAR1A antibody (STJ25122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PRKAR1A antibody (1-250) (STJ25122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using PKA I Alpha reg Polyclonal Antibody

Anti-PRKAR1A antibody (271-320 aa) (STJ95111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PRKAR1A antibody (300-386) (STJ11103928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PKA I Beta reg Polyclonal Antibody

Anti-PRKAR1B antibody (106-155 aa) (STJ95112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAR2A antibody (1-404) (STJ25123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using PKA RII Alpha (PRKAR2A) Rabbit monoclonal antibody (STJ11101964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRKAR2A antibody (312-404) [S4MR] (STJ11101964)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of 293 cells using PKA II Alpha reg Polyclonal Antibody

Anti-PRKAR2A antibody (41-90 aa) (STJ95109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat ovary using PRKAR2B Rabbit monoclonal antibody (STJ11103108) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PRKAR2B antibody (319-418) [S8MR] (STJ11103108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using PKA II Beta reg Polyclonal Antibody

Anti-PRKAR2B antibody (79-128 aa) (STJ95110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PRKC Alpha/Beta antibody (573-672 aa) (STJ11106944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using PKC alpha Rabbit monoclonal antibody (STJ11105041) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRKCA antibody (200-300 aa) [S5041RM] (STJ11105041)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-PKC (PTR1190) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 70kDaObserved band size: 80kDa

Anti-PRKCA antibody (450-550) [PTR1190] (STJA0006229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit/Bovine

£48.00 - £252.00

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Western blot analysis of various lysates, using PKC alpha Rabbit polyclonal antibody (STJ115305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRKCA antibody (573-672) (STJ115305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human colon using PKC alpha antibody (STJ25125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PRKCA antibody (573-672) (STJ25125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PKC alpha antibody (STJ112958). Western blot was performed from the immunoprecipitate using PKC alpha antibody (STJ112958) at a dilution of 1:1000.

Anti-PRKCA antibody (573-672) [S8MR] (STJ112958)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of NIH-3T3 cells using PKC Alpha Polyclonal Antibody

Anti-PRKCA antibody (606-655 aa) (STJ95119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PKC alpha antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-PRKCA antibody (658-672 aa) [R05-4E5] (STJA0013069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Anti-PRKCA antibody (Central region) [M237] (STJA0005531)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£394.50

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Anti-PRKCA antibody [M499] (STJA0005532)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Immunofluorescence analysis of HeLa cells using PKC alpha Rabbit monoclonal antibody (STJ11105054) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKCA antibody [S5054RM] (STJ11105054)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunocytochemistry analysis of PKC beta 2 (green) in K562 using PKC beta 2 antibody, and DAPI (blue).

Anti-PRKCB antibody (170-350 aa) [R06-0C2] (STJA0012652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat

£264.50

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Immunofluorescence analysis of NIH/3T3 cells using PKC-beta Rabbit polyclonal antibody (STJ29922) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKCB antibody (220-673) (STJ29922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using PKC-beta Rabbit polyclonal antibody (STJ115587) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKCB antibody (260-340) (STJ115587)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options