Autophagy | Research Area | St John's Laboratory

Immunofluorescence analysis of U-251MG cells using GFAP Rabbit polyclonal antibody (STJ116876) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GFAP antibody (350-432) (STJ116876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-GFAP antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-GFAP antibody (375aa C-Term) (STJ140132)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey

$182.93 - $532.58

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Immunohistochemistry of mouse brain with Anti-GFAP primary antibody (STJ501145) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GFAP antibody (50-100) (STJ501145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-GFAP antibody (50-100) {Biotin} (STJ501146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-GFAP antibody (50-100) {FITC} (STJ501147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Immunofluorescence analysis of Hela cell. 1, AR Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-GFAP antibody [5C8] (STJ96961)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-GFAP antibody [EP672Y] (STJ16100372)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$851.18 - $1,562.63

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Human brain stained with anti-GFAP antibody using peroxidase-conjugate and DAB chromogen. Note positive staining of astrocytic processes.

Anti-GFAP antibody [GA‐5] (STJ180110)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$218.03 - $772.88

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Anti-GFAP antibody [H8/E9] (STJA0019392)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$510.98 - $2,203.88

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Anti-GFAP antibody [N206B/9R] (STJA0019391)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/WB

Reactivity :

Human

$510.98 - $2,203.88

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Anti-GFAP antibody [SAA0567] (STJA0019390)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IHC/WB

Reactivity :

Human

$510.98 - $2,203.88

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Western blot analysis of GFAP at dilution of 1:1000

Anti-GFAP antibody [SM0401] (STJA0010401)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

$230.18 - $315.23

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Anti-GFAP antibody [SM0407] (STJA0010407)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

$230.18 - $315.23

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Anti-GFAP antibody [SM5031] (STJA0005031)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.23

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Anti-GFAP nanobody [SAA1221] (STJN000150)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IHC/WB

Reactivity :

Human

$510.98 - $2,203.88

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Anti-Glial Fibrillary Acidic Protein antibody [6F2] (STJ16100231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F/WB

Reactivity :

Human

$369.23

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Anti-Glial Fibrillary Acidic Protein antibody [GA5] (STJ16101820)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$1,829.93

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Anti-Glial Fibrillary Acidic Protein antibody [GF-01] (STJ16100238)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IP/WB/ICC

Reactivity :

Pig/Cat/Human

$655.43

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Western blot analysis of extracts of Raji cells, using HBB antibody (STJ111074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-HBB antibody (47-147) (STJ111074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HBB antibody (STJ192570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g HDAC6 antibody (STJ11103524). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ11103524) at a dilution of 1:1000.

Anti-HDAC6 antibody (1-100) [S4MR] (STJ11103524)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

$174.83 - $775.58

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using HDAC6 antibody (STJ23932) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC6 antibody (1-200) (STJ23932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HDAC6 (ABT-HDAC6) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 131kDaObserved band size: 130, 180kDa

Anti-HDAC6 antibody (1100-1215 aa) [ABT-HDAC6] (STJ196790)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse

$234.90 - $340.20

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Western blot analysis of various cells using Histone deacetylase 6 Polyclonal Antibody diluted at 1：2000

Anti-HDAC6 antibody (1166-1215 aa) (STJ93512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of U2OS cells using HDAC6 antibody (STJ113792).

Anti-HDAC6 antibody (160-470) (STJ113792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts from K-562 cells, using HDAC6 Rabbit monoclonal antibody (STJ11104740) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-HDAC6 antibody (600-700) [S4740RM] (STJ11104740)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Immunofluorescence analysis of Hela cell. 1, HDAC6 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HDAC6 antibody (7-56 aa) (STJ93483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunoprecipitation analysis of 100 Mu g extracts of HepG2 cells using 3 Mu g HDAC6 antibody (STJ113047). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ113047) at a dilution of 1:1000.

Anti-HDAC6 antibody (836-1104) (STJ113047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using HDAC6 Rabbit polyclonal antibody (STJ113748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HDAC6 antibody (836-1104) (STJ113748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-HDAC6 antibody [SM4991] (STJA0004991)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.23

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Western blot analysis of various cells using HSF1 Polyclonal Antibody diluted at 1：1000

Anti-HSF1 antibody (270-319 aa) (STJ93601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of U2OS cells using HSF1 Rabbit polyclonal antibody (STJ115712) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSF1 antibody (350-529) (STJ115712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded human colon using HSF1 Rabbit polyclonal antibody (STJ11104640) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-HSF1 antibody (367-529) (STJ11104640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of HSF1 in HeLa lysates using Hsf1 antibody.

Anti-HSF1 antibody (485-529 aa) [R09-3M8] (STJA0014242)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

$357.08

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HSF1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-HSF1 antibody (497-510 aa) [R07-0G0] (STJA0011457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

$332.78

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Anti-HSP70 antibody (STJ13100154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Marmoset/Rat/Mouse

$447.53

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Hsp90 Alpha antibody (STJ11102219). Western blot was performed from the immunoprecipitate using Hsp90 Alpha antibody (STJ11102219) at a dilution of 1:1000.

Anti-HSP90AA1 antibody (1-100) [S9MR] (STJ11102219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of various lysates, using HSP90AA1 Rabbit polyclonal antibody (STJ11105239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AA1 antibody (433-732 aa) (STJ11105239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using Hsp90 Alpha antibody (STJ115462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HSP90AA1 antibody (433-732) (STJ115462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using Hsp90 Alpha Rabbit polyclonal antibody (STJ24087). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-HSP90AA1 antibody (433-732) (STJ24087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of HSP90 in K562, C6, Hela lysates using Hsp90 alpha antibody.

Anti-HSP90AA1 antibody (62-75 aa) [R06-9C1] (STJA0010746)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$357.08

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Western blot analysis of various lysates using Hsp90 Alpha Rabbit pAb (STJ114322) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HSP90AA1 antibody (629-732) (STJ114322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSP90 a Mouse mAb diluted at 1:2, 000.

Anti-HSP90AA1 antibody (660-740 aa) [1F6] (STJ97496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of Hela cell. 1, HSP90A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP90AA1 antibody (683-732 aa) (STJ93622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot detection of Hsp90 in Hela, 3T3, C6, K562 and A549 cell lysates using Hsp90 mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.

Anti-HSP90AA1 antibody [4B6-G2-H6] (STJ99220)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$194.40 - $469.80

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Western blot detection of Hsp90 alpha in R Brain, Jurkat, CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Hsp90 alpha mouse mAb (1:1000 diluted). Predicted band size:90KDa. Observed band size:90KDa.

Anti-HSP90AA1 antibody [6H7-A3-2G6] (STJ99288)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

$194.40 - $469.80

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Immunofluorescence analysis of U2OS cells using Hsp90 Beta Rabbit polyclonal antibody (STJ11104548). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSP90AB1 antibody (1-300) (STJ11104548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from wild type (WT) and HSP90AB1 knockout (KO) HeLa cells, using [KO Validated] Hsp90 Beta Rabbit polyclonal antibody (STJ119988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AB1 antibody (1-300) (STJ119988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$174.83 - $867.38

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