• Rat Uchl1 (Ubiquitin carboxyl-terminal hydrolase isozyme L1) Sandwich ELISA (STJE0006430)

Rat Uchl1 (Ubiquitin carboxyl-terminal hydrolase isozyme L1) Sandwich ELISA (STJE0006430)

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STJE0006430

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This Uchl1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Uchl1
Gene ID: 29545
Uniprot ID: UCHL1_RAT
Specificity: This kit recognizes Rat UCHL1 in samples. No significant cross-reactivity or interference between Rat UCHL1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1
Uch-L1
Neuron Cytoplasmic Protein 9.5
Pgp 9.5
Pgp9.5
Ubiquitin Thioesterase L1
Database Links Reactome: R-RNO-5689603
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Lipid-Anchor
Alternative ELISA Names Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 ELISA kit
Uch-L1 ELISA kit
Neuron Cytoplasmic Protein 9.5 ELISA kit
Pgp 9.5 ELISA kit
Pgp9.5 ELISA kit
Ubiquitin Thioesterase L1 ELISA kit
Uchl1 ELISA kit
Specificity This kit recognizes Rat UCHL1 in samples. No significant cross-reactivity or interference between Rat UCHL1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-109 100
EDTA plasma (n=8) 93-108 100
Cell culture media (n=8) 84-100 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 200.02 430.69 1489.86 208.52 445.85 1600.11
Standard deviation 11.66 25.63 51.55 14.39 21.31 64.64
CV (%) 5.83 5.95 3.46 6.90 4.78 4.04
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance