• Mouse Uchl1 (Ubiquitin carboxyl-terminal hydrolase isozyme L1) Sandwich ELISA (STJE0005236)

Mouse Uchl1 (Ubiquitin carboxyl-terminal hydrolase isozyme L1) Sandwich ELISA (STJE0005236)

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STJE0005236

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This Uchl1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Uchl1
Gene ID: 22223
Uniprot ID: UCHL1_MOUSE
Specificity: This kit recognizes Mouse UCHL1 in samples. No significant cross-reactivity or interference between Mouse UCHL1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O-glycosylated.
Function Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes. Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis. Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators. Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins. Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1
Uch-L1
Neuron Cytoplasmic Protein 9.5
Pgp 9.5
Pgp9.5
Ubiquitin Thioesterase L1
Database Links Reactome: R-MMU-5689603
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Lipid-Anchor
Localizes Near And/Or Within Mitochondria To Potentially Interact With Mitochondrial Proteins
Alternative ELISA Names Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 ELISA kit
Uch-L1 ELISA kit
Neuron Cytoplasmic Protein 9.5 ELISA kit
Pgp 9.5 ELISA kit
Pgp9.5 ELISA kit
Ubiquitin Thioesterase L1 ELISA kit
Uchl1 ELISA kit
Specificity This kit recognizes Mouse UCHL1 in samples. No significant cross-reactivity or interference between Mouse UCHL1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 96
EDTA plasma (n=8) 89-102 96
Cell culture media (n=8) 93-106 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 193.96 439.24 1375.45 187.35 410.81 1363.59
Standard deviation 12.59 22.93 42.91 11.63 16.97 69.68
CV (%) 6.49 5.22 3.12 6.21 4.13 5.11
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance