| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This Uchl1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Uchl1 |
| Gene ID: |
29545 |
| Uniprot ID: |
UCHL1_RAT |
| Specificity: |
This kit recognizes Rat UCHL1 in samples. No significant cross-reactivity or interference between Rat UCHL1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1Uch-L1Neuron Cytoplasmic Protein 9.5Pgp 9.5Pgp9.5Ubiquitin Thioesterase L1 |
| Database Links | Reactome: R-RNO-5689603 |
| Cellular Localisation | CytoplasmEndoplasmic Reticulum MembraneLipid-Anchor |
| Alternative ELISA Names | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 ELISA kitUch-L1 ELISA kitNeuron Cytoplasmic Protein 9.5 ELISA kitPgp 9.5 ELISA kitPgp9.5 ELISA kitUbiquitin Thioesterase L1 ELISA kitUchl1 ELISA kit |
| Specificity | This kit recognizes Rat UCHL1 in samples. No significant cross-reactivity or interference between Rat UCHL1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 100 |
| EDTA plasma (n=8) | 93-108 | 100 |
| Cell culture media (n=8) | 84-100 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 200.02 | 430.69 | 1489.86 | 208.52 | 445.85 | 1600.11 |
| Standard deviation | 11.66 | 25.63 | 51.55 | 14.39 | 21.31 | 64.64 |
| CV (%) | 5.83 | 5.95 | 3.46 | 6.90 | 4.78 | 4.04 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
