| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This Tf Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Tf |
| Gene ID: |
24825 |
| Uniprot ID: |
TRFE_RAT |
| Specificity: |
This kit recognizes Rat TF in samples. No significant cross-reactivity or interference between Rat TF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. |
| Protein Name | SerotransferrinTransferrinBeta-1 Metal-Binding GlobulinLiver Regeneration-Related Protein Lrrg03Siderophilin |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-381426Reactome: -RNO-8856825Reactome: -RNO-8856828Reactome: -RNO-8957275Reactome: -RNO-917937Reactome: -RNO-917977 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Serotransferrin ELISA kitTransferrin ELISA kitBeta-1 Metal-Binding Globulin ELISA kitLiver Regeneration-Related Protein Lrrg03 ELISA kitSiderophilin ELISA kitTf ELISA kit |
| Specificity | This kit recognizes Rat TF in samples. No significant cross-reactivity or interference between Rat TF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 101-109 | 105 |
| EDTA plasma (n=8) | 88-100 | 92 |
| Cell culture media (n=8) | 91-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 160.09 | 440.88 | 1702.97 | 160.83 | 442.91 | 1988.89 |
| Standard deviation | 9.61 | 20.28 | 92.13 | 8.91 | 20.46 | 80.95 |
| CV (%) | 6.00 | 4.60 | 5.41 | 5.54 | 4.62 | 4.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
