| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat TACR1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Tacr1 |
| Gene ID: |
24807 |
| Uniprot ID: |
NK1R_RAT |
| Specificity: |
This kit recognizes Rat TACR1 in samples. No significant cross-reactivity or interference between Rat TACR1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. |
| Protein Name | Substance-P ReceptorSprNk-1 ReceptorNk-1rTachykinin Receptor 1 |
| Database Links | Reactome: R-RNO-380095Reactome: -RNO-416476Reactome: -RNO-8856825Reactome: -RNO-8856828 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | Substance-P Receptor CLIA kitSpr CLIA kitNk-1 Receptor CLIA kitNk-1r CLIA kitTachykinin Receptor 1 CLIA kitTacr1 CLIA kitTac1r CLIA kit |
| Specificity | This kit recognizes Rat TACR1 in samples. No significant cross-reactivity or interference between Rat TACR1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-113 | 106 |
| EDTA plasma(n=8) | 83-95 | 90 |
| Cell culture media(n=8) | 94-110 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 108.21 | 295.83 | 708.96 | 117.30 | 278.85 | 662.17 |
| Standard deviation | 12.83 | 26.15 | 73.38 | 14.39 | 19.58 | 59.53 |
| CV (%) | 11.86 | 8.84 | 10.35 | 12.27 | 7.02 | 8.99 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
