• Rat Syp (Synaptophysin) Sandwich ELISA (STJE0006127)

Rat Syp (Synaptophysin) Sandwich ELISA (STJE0006127)

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STJE0006127

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description: This Syp Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Syp
Gene ID: 24804
Uniprot ID: SYPH_RAT
Specificity: This kit recognizes Rat SYP in samples. No significant cross-reactivity or interference between Rat SYP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. Phosphorylated by SRC.
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Protein Name Synaptophysin
Major Synaptic Vesicle Protein P38
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Multi-Pass Membrane Protein
Synapse
Synaptosome
Alternative ELISA Names Synaptophysin ELISA kit
Major Synaptic Vesicle Protein P38 ELISA kit
Syp ELISA kit
Specificity This kit recognizes Rat SYP in samples. No significant cross-reactivity or interference between Rat SYP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 96
EDTA plasma (n=8) 88-102 94
Cell culture media (n=8) 87-99 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.88 5.79 16.42 2.06 6.19 15.32
Standard deviation 0.10 0.28 0.66 0.14 0.33 0.74
CV (%) 5.32 4.84 4.02 6.80 5.33 4.83
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance