| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.144ng/mL |
| Detection Limit: |
0.39-25ng/mL |
| Short Description: |
This SREBF1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Srebf1 |
| Gene ID: |
78968 |
| Uniprot ID: |
SRBP1_RAT |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed predominantly in brown adipose tissue. |
| Post Translational Modifications | Sterol regulatory element-binding protein 1: Processed in the Golgi apparatus, releasing the protein from the membrane. At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum. In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-395 by SIK1 represses activity possibly by inhibiting DNA-binding. Processed sterol regulatory element-binding protein 1: Ubiquitinated.the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus. |
| Function | Sterol regulatory element-binding protein 1: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis. Processed sterol regulatory element-binding protein 1: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation. Isoform SREBP-1A: Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C. Able to stimulate both lipogenic and cholesterogenic gene expression. Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver. Required for innate immune response in macrophages by regulating lipid metabolism. Isoform SREBP-1C: Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A. Primarily controls expression of lipogenic gene. Strongly activates global lipid synthesis in rapidly growing cells. |
| Protein Name | Sterol Regulatory Element-Binding Protein 1Srebp-1Adipocyte Determination- And Differentiation-Dependent Factor 1Add1Sterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1Transcription Factor Srebf1 |
| Database Links | Reactome: R-RNO-1655829Reactome: -RNO-191273 |
| Cellular Localisation | Sterol Regulatory Element-Binding Protein 1: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCytoplasmic VesicleCopii-Coated Vesicle MembraneAt High Sterol ConcentrationsThe Scap-Srebp Is Retained In The Endoplasmic ReticulumLow Sterol Concentrations Promote Recruitment Into Copii-Coated Vesicles And Transport Of The Scap-Srebp To The GolgiWhere It Is ProcessedProcessed Sterol Regulatory Element-Binding Protein 1: Nucleus |
| Alternative ELISA Names | Sterol Regulatory Element-Binding Protein 1 ELISA kitSrebp-1 ELISA kitAdipocyte Determination- And Differentiation-Dependent Factor 1 ELISA kitAdd1 ELISA kitSterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1 ELISA kitTranscription Factor Srebf1 ELISA kitSrebf1 ELISA kitSrebp1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
