| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This SREBP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SREBF1 |
| Gene ID: |
6720 |
| Uniprot ID: |
SRBP1_HUMAN |
| Specificity: |
This kit recognizes Human SREBP in samples. No significant cross-reactivity or interference between Human SREBP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Sterol regulatory element-binding protein 1: Processed in the Golgi apparatus, releasing the protein from the membrane. At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum. In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding. Processed sterol regulatory element-binding protein 1: Ubiquitinated.the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus. |
| Function | Sterol regulatory element-binding protein 1: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis. Processed sterol regulatory element-binding protein 1: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation. Isoform SREBP-1A: Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C. Able to stimulate both lipogenic and cholesterogenic gene expression. Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver. Required for innate immune response in macrophages by regulating lipid metabolism. Isoform SREBP-1C: Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A. Primarily controls expression of lipogenic gene. Strongly activates global lipid synthesis in rapidly growing cells. Isoform SREBP-1aDelta: The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters. Isoform SREBP-1cDelta: The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters. |
| Protein Name | Sterol Regulatory Element-Binding Protein 1Srebp-1Class D Basic Helix-Loop-Helix Protein 1Bhlhd1Sterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1Transcription Factor Srebf1 |
| Database Links | Reactome: R-HSA-1368082Reactome: R-HSA-1655829Reactome: R-HSA-191273Reactome: R-HSA-1989781Reactome: R-HSA-2426168Reactome: R-HSA-381340 P36956-1Reactome: R-HSA-9029558 P36956-3Reactome: R-HSA-9615017 |
| Cellular Localisation | Sterol Regulatory Element-Binding Protein 1: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCytoplasmic VesicleCopii-Coated Vesicle MembraneAt High Sterol ConcentrationsThe Scap-Srebp Is Retained In The Endoplasmic ReticulumLow Sterol Concentrations Promote Recruitment Into Copii-Coated Vesicles And Transport Of The Scap-Srebp To The GolgiWhere It Is ProcessedProcessed Sterol Regulatory Element-Binding Protein 1: NucleusIsoform Srebp-1adelta: NucleusIsoform Srebp-1cdelta: Nucleus |
| Alternative ELISA Names | Sterol Regulatory Element-Binding Protein 1 ELISA kitSrebp-1 ELISA kitClass D Basic Helix-Loop-Helix Protein 1 ELISA kitBhlhd1 ELISA kitSterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1 ELISA kitTranscription Factor Srebf1 ELISA kitSREBF1 ELISA kitBHLHD1 ELISA kitSREBP1 ELISA kit |
| Specificity | This kit recognizes Human SREBP in samples. No significant cross-reactivity or interference between Human SREBP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-100 | 93 |
| EDTA plasma (n=8) | 96-108 | 102 |
| Cell culture media (n=8) | 87-100 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.95 | 2.38 | 8.55 | 1.04 | 2.52 | 8.38 |
| Standard deviation | 0.05 | 0.13 | 0.47 | 0.05 | 0.13 | 0.32 |
| CV (%) | 5.26 | 5.46 | 5.50 | 4.81 | 5.16 | 3.82 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
