| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This Slc2a4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Slc2a4 |
| Gene ID: |
25139 |
| Uniprot ID: |
GLUT4_RAT |
| Specificity: |
This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or interference between Rat GLUT4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Sumoylated. Palmitoylated. Palmitoylation by ZDHHC7 controls the insulin-dependent translocation of GLUT4 to the plasma membrane. |
| Function | Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell. |
| Protein Name | Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4Glucose Transporter Type 4 - Insulin-ResponsiveGlut-4 |
| Database Links | Reactome: R-RNO-189200 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndomembrane SystemCytoplasmPerinuclear RegionLocalizes Primarily To The Perinuclear RegionUndergoing Continued Recycling To The Plasma Membrane Where It Is Rapidly ReinternalizedThe Dileucine Internalization Motif Is Critical For Intracellular SequestrationInsulin Stimulation Induces Translocation To The Cell Membrane |
| Alternative ELISA Names | Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 ELISA kitGlucose Transporter Type 4 - Insulin-Responsive ELISA kitGlut-4 ELISA kitSlc2a4 ELISA kitGlut4 ELISA kit |
| Specificity | This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or interference between Rat GLUT4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-101 | 96 |
| EDTA plasma (n=8) | 88-101 | 94 |
| Cell culture media (n=8) | 83-98 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.03 | 4.50 | 19.63 | 2.21 | 4.26 | 19.21 |
| Standard deviation | 0.11 | 0.21 | 0.98 | 0.13 | 0.18 | 0.75 |
| CV (%) | 5.42 | 4.67 | 4.99 | 5.88 | 4.23 | 3.90 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
