| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This rat SHBG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Shbg |
| Gene ID: |
24775 |
| Uniprot ID: |
SHBG_RAT |
| Specificity: |
This kit recognizes Rat SHBG in samples. No significant cross-reactivity or interference between Rat SHBG and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 2 is only expressed in the liver. |
| Function | Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. |
| Protein Name | Sex Hormone-Binding GlobulinShbgSex Steroid-Binding ProteinSbpTestis-Specific Androgen-Binding ProteinAbp |
| Cellular Localisation | SecretedIn TestisIt Is Synthesized By The Sertoli CellsSecreted Into The Lumen Of The Seminiferous Tubule And Transported To The Epididymis |
| Alternative CLIA Names | Sex Hormone-Binding Globulin CLIA kitShbg CLIA kitSex Steroid-Binding Protein CLIA kitSbp CLIA kitTestis-Specific Androgen-Binding Protein CLIA kitAbp CLIA kitShbg CLIA kit |
| Specificity | This kit recognizes Rat SHBG in samples. No significant cross-reactivity or interference between Rat SHBG and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-105 | 97 |
| EDTA plasma(n=8) | 98-113 | 106 |
| Cell culture media(n=8) | 97-110 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 27.33 | 56.19 | 182.20 | 26.65 | 53.92 | 165.29 |
| Standard deviation | 3.32 | 3.97 | 11.02 | 2.72 | 4.23 | 18.38 |
| CV (%) | 12.15 | 7.07 | 6.05 | 10.21 | 7.84 | 11.12 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
