• Mouse Shbg (Sex hormone-binding globulin) Sandwich ELISA (STJE0004964)

Mouse Shbg (Sex hormone-binding globulin) Sandwich ELISA (STJE0004964)

SKU:
STJE0004964

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This Shbg Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Shbg
Gene ID: 20415
Uniprot ID: SHBG_MOUSE
Specificity: This kit recognizes Mouse SHBG in samples. No significant cross-reactivity or interference between Mouse SHBG and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Sex Hormone-Binding Globulin
Shbg
Sex Steroid-Binding Protein
Sbp
Testis-Specific Androgen-Binding Protein
Abp
Cellular Localisation Secreted
In Testis
It Is Synthesized By The Sertoli Cells
Secreted Into The Lumen Of The Seminiferous Tubule And Transported To The Epididymis
Alternative ELISA Names Sex Hormone-Binding Globulin ELISA kit
Shbg ELISA kit
Sex Steroid-Binding Protein ELISA kit
Sbp ELISA kit
Testis-Specific Androgen-Binding Protein ELISA kit
Abp ELISA kit
Shbg ELISA kit
Specificity This kit recognizes Mouse SHBG in samples. No significant cross-reactivity or interference between Mouse SHBG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-105 97
EDTA plasma (n=8) 90-105 96
Cell culture media (n=8) 90-102 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 100.00 273.63 767.14 104.15 266.24 693.02
Standard deviation 5.67 12.09 23.01 5.45 10.73 28.55
CV (%) 5.67 4.42 3.00 5.23 4.03 4.12
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance