| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This Sftpd Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Sftpd |
| Gene ID: |
25350 |
| Uniprot ID: |
SFTPD_RAT |
| Specificity: |
This kit recognizes Rat SP-D in samples. No significant cross-reactivity or interference between Rat SP-D and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. |
| Protein Name | Pulmonary Surfactant-Associated Protein DPsp-DSp-DCp4Lung Surfactant Protein D |
| Database Links | Reactome: R-RNO-198933Reactome: -RNO-391160Reactome: -RNO-5683826Reactome: -RNO-5686938 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixSurface Film |
| Alternative ELISA Names | Pulmonary Surfactant-Associated Protein D ELISA kitPsp-D ELISA kitSp-D ELISA kitCp4 ELISA kitLung Surfactant Protein D ELISA kitSftpd ELISA kitSftp4 ELISA kit |
| Specificity | This kit recognizes Rat SP-D in samples. No significant cross-reactivity or interference between Rat SP-D and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-101 | 96 |
| EDTA plasma (n=8) | 94-107 | 101 |
| Cell culture media (n=8) | 94-109 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 252.07 | 590.57 | 2301.76 | 247.61 | 552.21 | 2378.08 |
| Standard deviation | 16.28 | 25.10 | 90.00 | 15.08 | 33.08 | 101.07 |
| CV (%) | 6.46 | 4.25 | 3.91 | 6.09 | 5.99 | 4.25 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
