| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This rat SELE kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Sele |
| Gene ID: |
25544 |
| Uniprot ID: |
LYAM2_RAT |
| Specificity: |
This kit recognizes Rat SELE in samples. No significant cross-reactivity or interference between Rat SELE and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis. |
| Protein Name | E-SelectinCd62 Antigen-Like Family Member EEndothelial Leukocyte Adhesion Molecule 1Elam-1Leukocyte-Endothelial Cell Adhesion Molecule 2Lecam2Cd Antigen Cd62e |
| Database Links | Reactome: R-RNO-202733 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | E-Selectin CLIA kitCd62 Antigen-Like Family Member E CLIA kitEndothelial Leukocyte Adhesion Molecule 1 CLIA kitElam-1 CLIA kitLeukocyte-Endothelial Cell Adhesion Molecule 2 CLIA kitLecam2 CLIA kitCd Antigen Cd62e CLIA kitSele CLIA kitElam-1 CLIA kit |
| Specificity | This kit recognizes Rat SELE in samples. No significant cross-reactivity or interference between Rat SELE and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-108 | 99 |
| EDTA plasma(n=8) | 91-105 | 96 |
| Cell culture media(n=8) | 95-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 48.02 | 91.82 | 424.17 | 44.89 | 89.53 | 397.69 |
| Standard deviation | 4.33 | 6.45 | 43.01 | 4.31 | 6.28 | 37.34 |
| CV (%) | 9.02 | 7.02 | 10.14 | 9.60 | 7.01 | 9.39 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
