• Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit (STJC0001872)

Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit (STJC0001872)

SKU:
STJC0001872

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This rat RUNX2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Runx2Uniprot ID=RUNX2_RAT"
Specificity: This kit recognizes Rat RUNX2 in samples. No significant cross-reactivity or interference between Rat RUNX2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation.
Function Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
Protein Name Runt-Related Transcription Factor 2
Core-Binding Factor Subunit Alpha-1
Cbf-Alpha-1
Osteoblast-Specific Transcription Factor 2
Osf-2
Cellular Localisation Nucleus
Cytoplasm
Alternative CLIA Names Runt-Related Transcription Factor 2 CLIA kit
Core-Binding Factor Subunit Alpha-1 CLIA kit
Cbf-Alpha-1 CLIA kit
Osteoblast-Specific Transcription Factor 2 CLIA kit
Osf-2 CLIA kit
Runx2 CLIA kit
Cbfa1 CLIA kit
Osf2 CLIA kit
Specificity This kit recognizes Rat RUNX2 in samples. No significant cross-reactivity or interference between Rat RUNX2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-111 104
EDTA plasma(n=8) 92-105 100
Cell culture media(n=8) 86-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 192.14 626.83 1569.76 187.48 569.38 1437.64
Standard deviation 19.16 51.15 106.43 22.89 47.03 98.62
CV (%) 9.97 8.16 6.78 12.21 8.26 6.86
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance